Detection of thymine [2+2] photodimer repair in DNA: selective reaction of KMnO4.
نویسندگان
چکیده
The specific reaction of potassium permanganate with thymine in single-stranded DNA was employed to analyze thymine [2+2] dimer repair in DNA and in DNA/peptide nucleic acid hybrid duplexes. This simple and highly sensitive chemical assay is convenient for monitoring repair of thymine dimers in oligonucleotides.
منابع مشابه
Enzymatic reaction with unnatural substrates: DNA photolyase (Escherichia coli) recognizes and reverses thymine [2+2] dimers in the DNA strand of a DNA/PNA hybrid duplex.
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ورودعنوان ژورنال:
- Nucleic acids research
دوره 26 17 شماره
صفحات -
تاریخ انتشار 1998